Synthetic Nucleic Acids as Inhibitors of Gene Expression Mechanisms, Applications, and Therapeutic Implications by Levon Michael Khachigian

Cover of: Synthetic Nucleic Acids as Inhibitors of Gene Expression | Levon Michael Khachigian

Published by CRC .

Written in English

Read online


  • Genetics (non-medical),
  • Science/Mathematics,
  • Life Sciences - Genetics & Genomics,
  • Science,
  • Science / Molecular Biology,
  • Life Sciences - Biology - Molecular Biology,
  • Catalytic RNA,
  • Genetic regulation,
  • Repressors, Genetic

Book details

The Physical Object
Number of Pages200
ID Numbers
Open LibraryOL8259931M
ISBN 100849330254
ISBN 109780849330254

Download Synthetic Nucleic Acids as Inhibitors of Gene Expression

Synthetic Nucleic Acids as Inhibitors of Gene Expression: Mechanisms, Applications, and Therapeutic Implications brings together timely, authoritative reviews by renowned international leaders in the fields of ribozymes, RNA interference, DNAzymes and oligonucleotide decoys, with emphasis on biochemistry and translational : Hardcover.

Synthetic Nucleic Acids as Inhibitors of Gene Expression book. Synthetic Nucleic Acids as Inhibitors of Gene Expression book. Mechanisms, Applications, and Therapeutic Implications. Edited By Levon Michael Khachigian. Edition 1st Edition.

First Published eBook Published 29 November Cited by: 3. Synthetic nucleic acids as inhibitors of gene expression: mechanisms, applications, and therapeutics implications.

[Levon Khachigian;] -- "This book brings together timely, authoritative reviews by renowned international leaders in the fields of ribozymes, RNA interference, DNAzymes and oligonucleotide decoys, with emphasis on. Synthetic nucleic acids as inhibitors of gene expression: mechanisms, applications, and therapeutics implications.

Synthetic nucleic acids are at the cutting-edge of molecular strategies that target genes of interest in a specific, efficient and cost-effective manner. These tools are now used as gene-silencing agents in vitro and as novel therapeutics in human disease.

This book brings together reviews by leaders in the fields of ribozymes, RNA interference. Other chapters provide a critical assessment of eukaryotic nucleic acid polymerases. This book discusses as well some examples from plant mitochondrial systems. The final chapter deals with two special areas of plant biology where the expression of the nucleic acids is seen in striking relief, the formation of plant tumors, and the growth and.

In this study, S. pyogenes growth was inhibited by application of peptide-conjugated antisense-peptide nucleic acids (PNAs) specific for the essential gyrase A gene (gyrA). Thereby, HIV-1 Tat peptide-coupled PNAs were more efficient inhibitors of streptococcal growth as compared with (KFF)3K-coupled PNAs.

Antisense oligonucleotides and short interfering RNAs (siRNAs) are nucleic acids-targeting reagents for gene expression modulation that are being developed as drugs for many applications.

A number of useful synthetic nucleic acids analogues have been introduced recently to greatly improve their properties for use as therapeutics. While Streptococcus pyogenes is consistently susceptible toward penicillin, therapeutic failure of penicillin treatment has been reported repeatedly and a considerable number of patients exhibit allergic reactions to this substance.

At the same time, streptococcal resistance to alternative antibiotics, e.g., macrolides, has increased. Taken together, these facts demand the development of novel.

DNA, the fundamental genetic polymer of all living organisms on Earth, can be chemically modified to embrace novel functions that do not exist in nature.

Synthetic Nucleic Acids as Inhibitors of Gene Expression book The key chemical and structural parameters for genetic information storage, heredity, and evolution have been elucidated, and many xenobiotic nucleic acids (XNAs) with non-canonical structures are developed as alternative genetic materials in.

A milestone for the origin of life was the onset of a sustained functional interplay between nucleic acids, peptides, lipids and sugars, to form chemical dynamic off-equilibrium systems. Marcus-Sekura CJ, Woerner AM, Shinozuka K, Zon G, Quinnan GV () Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkage.

Nucleic Acids Res – PubMed Google Scholar. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation (i.e., RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

Antisense oligonucleotides and short interfering RNAs (siR-NAs) are nucleic acids-targeting reagents for gene expression modulation that are being developed as drugs for many applications.

Keywords:gene expression, antisense oligonucleotides, dna mimetics, intercalators, photoreactive agents, antigene Abstract: Artificial control of gene expression has great potential in the treatment of many human diseases, and peptide nucleic acids (PNAs) offer several potential advantages for silencing gene expression in mammalian cells.

Among these nanomedicines, nanoparticle mediated delivery of nucleic acids has been put forward as key instrument to modulate gene expression, be it targeted gene silencing, interference RNA mechanisms and/or gene edition. These novel delivery systems have strongly relied on nanoparticles and, in particular, gold nanoparticles (AuNPs) have.

Design of antisense peptide nucleic acids (PNAs) specific for gyrA in GAS M Upper case: partial sequence of the gyrase A gene, start-codon marked in bold; lower case, respective PNA sequences, e.g., 8-amino-3,6-dioxaoctanoic acid.(a) (KFF)3K-coupled PNAs complementary to the start-codon region of gyrA.(b) (KFF)3K-coupled PNAs composed of the same bases as in A but in a.

Fatty Acid Synthesis Inhibitors Nucleic Acid Synthesis Inhibitors Cerulenin Nucleic Acids Uridine RNA Protein Synthesis Inhibitors Thymidine Phosphorus Isotopes DNA Tritium Carbon Isotopes RNA, Bacterial Peptide Nucleic Acids Uracil Hypoxanthines Cycloheximide Adenine Purines DNA, Bacterial Anisomycin Fatty Acids Emetine Puromycin Dactinomycin.

Publication Date range begin – Publication Date range end. Current results range from to View distribution.

The MagMAX Total Nucleic Acid Isolation Kit is designed for the rapid, high-throuphput purification of RNA and genomic DNA from a variety of samples including viral, blood, and bacterial samples.

It includes zirconia beads for the mechanical disruption of difficult pathogens. RNA purified using the. USB1 US09/, USA USB1 US B1 US B1 US B1 US A US A US A US B1 US B1 US B1 Authority US United States Prior art keywords nucleic acid nucleic acids sequences splicing gene Prior art date Legal status (The legal status is an assumption and is not a legal.

mitfa(b) embryos were microinjected with rCas9, synthetic gRNA, and synthetic DNA templates designed to restore the wild-type mitfa sequence and gene function. Microinjected embryos were scored at 48hpf for phenotypic evidence of melanocytes, indicative.

where is the expected value for the hypergeometric distribution. When k is more (or less) than expected, we calculate the probability of observing k or greater (or fewer) events. In our application, N is the total number of genes in the ranked lists, s and M are the rank thresholds and k is the number of overlapping genes (shown schematically in Figure 1 A).

Microbiosci is committed to providing you with a full array of target genome editing service for food additives production according customer‘s requests. Tell us how we can help you today.

Noble SA, Bonham MA, Bisi JE et al. Impact of biophysical parameters on the biological assessment of peptide nucleic acids, antisense inhibitors of gene expression. Drug Development Research ; – CrossRef Google Scholar. Peptide nucleic acid (PNA) analogues of DNA have attracted interest as potential pharmacological regulators of gene expression since they have the capacity to invade duplex DNA forming Watson−Crick base paired PNA:DNA heteroduplexes.

Unfortunately, strand invasion is limited to homopurine and homopyrimidine sequences and there is the need to explore further PNA analogues for the purpose of.

Progress has been made in the development of different types of nucleic acids such as DNA and siRNA with the potential to form the basis of new treatments for genetic and acquired disorders.

The lack of suitable vectors for the delivery of nucleic acids, however, represents a major hurdle to their continued development and therapeutic application. Gene design and splicing efficiency analysis of artificial rbcS2i1 insertion sites in the shble coding sequence represented by the transformation efficiency of C.

reinhardtii.(A) Antibiotic selection cassette adopted from the pOptimized vector toolkit containing the shble gene and an N-terminal linker which includes an additional SmaI restriction enzyme cleavage site and a representative TAP.

Abstract: Improving the performance of non-viral gene-delivery vehicles that consist of synthetic compounds and nucleic acids is a key to successful gene therapy. Supplementing synthetic vehicles with various biological functions by using natural or artificial peptides is a promising approach with which to achieve this goal.

Solid Phase Peptide Synthesis (SPPS) The control and regulatory mechanisms for many biological processes are dependent on peptides and proteins derived from α–amino acids.

In addition, many modern medicines are now produced from peptides or derivatives of peptides. Bridged nucleic acids (BNAs) are artificial bicyclic oligonucleotides that contain a five-membered or six-membered bridged structure with a “fixed” C3’-endo sugar puckering (Saenger ).

The bridge is synthetically incorporated at the 2’, 4’-position of the ribose to afford a 2’, 4’-BNA monomer. NIH GUIDELINES FOR RESEARCH INVOLVING RECOMBINANT OR SYNTHETIC NUCLEIC ACID MOLECUL ES (NIH GUIDELINES) APRIL DEPARTMENT OF HEALTH AND HUMAN SERVICES National Institutes of H.

Chemical Biology and Drug DesignNucleic Acids ResearchNucleic Acid TherapeuticsArtificial DNAMethodsScientific Reports. U1 snRNP (U1 small nuclear ribonucleoprotein) is an essential component of the splicing machinery. U1 snRNP also plays an additional role in 3′-end mRNA processing when it binds close to.

Molecular Cloning: A Laboratory Manual (Fourth Edition)Molecular Cloning has served as the foundation of technical expertise in labs worldwide for 30 other manual has been so popular, or so influential. Molecular Cloning, Fourth Edition, by the celebrated founding author Joe Sambrook and new co-author, the distinguished HHMI investigator Michael Green, preserves the highly praised.

Biochemistry All Publications/Website. OR SEARCH CITATIONS. Nucleic Acids Research, 39(1) VanderMeijden, B; Robinson, J A (). Synthesis and application of photoproline - a photoactivatable derivative of proline.

Nucleic acid metabolism is the process by which nucleic acids (DNA and RNA) are synthesized and degraded. Nucleic acids are Nucleic Acids Book (free online book on the chemistry and biology of nucleic acids) Interactive overview of nucleic acid Destruction of nucleic acid is a catabolic reaction.

Additionally, parts of the nucleotides or. A novel source for miR expression through the alternative polyadenylation of VMP1 gene transcripts. Nucleic Acids Res. Aug 1;40(14) PMCID: PMC (*equal contribution) Lupold SE, Johnson T, Chowdhury WH, and Rodriguez R. A real time Metridia Luciferase based non-invasive reporter assay of mammalian cell viability and.

Nucleic Acids Research, ISSN02/, Vol Issue 4, pp. The repeating, or monomer, units that are linked together to form nucleic acids are known as nucleotides A monomer unit that is linked together to form nucleic deoxyribonucleic acid (DNA) of a typical mammalian cell contains about 3 × 10 9 nucleotides.

Nucleotides can be further broken down to phosphoric acid (H 3 PO 4), a pentose sugar (a sugar with five carbon atoms), and a.Synthetic lethality. Synthetic lethality arises when a combination of deficiencies in the expression of two or more genes leads to cell death, whereas a deficiency in only one of these genes does not.

The deficiencies can arise through mutation, epigenetic alteration or by inhibition of a gene's expression.nucleic acids as detection tools bacterial riboswitch discovery and analysis The Chemical Biology of Nucleic Acids is an essential compendium of the synthesis of nucleic acids and their biological applications for bioorganic chemists, chemical biologists, medicinal chemists, cell biologists, and molecular biologists.

72492 views Tuesday, November 24, 2020